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anti p ikk  (Cell Signaling Technology Inc)


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    Structured Review

    Cell Signaling Technology Inc anti p ikk
    Anti P Ikk, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1253 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/p+psd95/PSD95+Antibody/pm41274095-79-19-21
    Average 96 stars, based on 1253 article reviews
    anti p ikk - by Bioz Stars, 2026-09
    96/100 stars

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    Related Articles

    Concentration Assay:

    Article Title: The effects of different intensities of aerobic exercise for 8 weeks on neurogenesis, depression, and anxiety in young mice
    Article Snippet: For immunodetection, the membranes were blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween-20 (TBS-T) at room temperature for one hour, followed by an overnight incubation at 4°C with primary antibodies.in Tris-buffered saline with 0.1% Tween-20 (TBS-T) at room temperature for one hour, followed by an overnight incubation at 4°C with primary antibodies. ... The primary antibodies used were: p-Akt (1:1,000, #4058), total Akt (1:1,000, #4691), p-ERK1/2 (1:3,000, #4370), total ERK1/2 (1:3,000, #9102), p-CREB (1:1,000, #9198), total CREB (1:1,000, #9197), p-AMPK (1:1,000, #2537), total AMPK (1:1,000, #2532), p-PSD95 (1:1,000, #45737), and total PSD95 (1:1,000, #3409) from Cell Signaling Technology (Danvers, MA, USA); p-Synapsin1 (S9, 1:1,000, ab76260), p-Synapsin1 (S549, 1:1,000, ab254035), p-Synapsin1 (S603, 1:1,000, ab13879), total Synapsin1 (1:1,000, ab64581), and DCX (1:1,000, ab18723) from Abcam (Cambridge, MA); and β-actin (1:30,000, #A1978) from Sigma-Aldrich.. After three 10- min washes with TBS-T at room temperature, the membranes were incubated with secondary antibodies conjugated to horseradish peroxidase, either goat anti-rabbit (Pierce, Rockford, IL, USA) or anti-mouse (Thermo Scientific; 31430).After three 10- min washes with TBS-T at room temperature, the membranes were incubated with secondary antibodies conjugated to horseradish peroxidase, either goat..

    Article Title: Intranasal Administration of Melanin-Concentrating Hormone Reduces Stress-Induced Anxiety- and Depressive-Like Behaviors in Rodents
    Article Snippet: For immunodetection, the blots were blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) for 1 h at room temperature and incubated overnight at 4°C with primary antibodies.For immunodetection, the blots were blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) for 1 h at room temperature and incubated overnight at 4°C with primary antibodies.. The following primary antibodies were used: p-Akt (1:1,000, #4058), total Akt (1:1,000, #4691), p-ERK1/2 (1:3,000, #4370), total ERK1/2 (1:3,000, #9102), p-mTOR (1:500, #2971), total mTOR (1:500, #2983), p-p70S6K (1:500, #9204), p-PSD95 (1:1,000, #45737), total PSD95 (1:1,000, #3409), p-GluR1 (1:1,000, #8084), total GluR1 (1:500, #13185), p-Synapsin1 (p-Syn1, 1:1,000, #88246), and total Syn1 (1:1,000, #9297) from Cell Signaling Technology (Danvers, MA, USA) and β-actin (1:30,000, #A1978) from Sigma-Aldrich.. After washing with TBS-T three times (10 min per wash) at room temperature, the membrane was incubated with secondary horseradish peroxidase-conjugated goat anti-rabbit (Pierce, Rockford, IL, USA) or anti-mouse (Thermo Scientific; 31430) antibodies.After washing with TBS-T three times (10 min per wash) at room temperature, the membrane was incubated with secondary horseradish peroxidase-conjugated goat anti-rabbit (Pierce, Rockford, IL, USA) or anti-mouse (Thermo Scientific;..

    Article Title: Acupuncture modulates stress response by the mTOR signaling pathway in a rat post-traumatic stress disorder model
    Article Snippet: The membrane was blocked in 5% skim milk in Tris buffered saline containing 0.1% Tween-20 (TBS-T) and incubated with the primary antibodies overnight at 4 °C.antibodies overnight at 4 °C. ... The primary antibodies: p-Akt (1:1000, Cell Signaling Technology, #4058), total Akt (1:1000, Cell Signaling Technology, #4691), p-ERK1/2 (1:3000, Cell Signaling Technology, #4370), total ERK1/2 (1:3000, Cell Signaling Technology, #9102), p-mTOR (1:500, Cell Signaling Technology, #2971), total mTOR (1:500, Cell Signaling Technology, #2983), p-CREB (1:500, Cell Signaling Technology, #9198), total CREB (1:500, Cell Signaling Technology, #9197), p-4E-BP-1 (1:500, Cell Signaling Technology, #9456), p-p70S6K (1:500, Cell Signaling Technology, #9204), p-Syn1 (1:500, Cell Signaling Technology, #88246), p-GluR1 (1:500, Cell Signaling Technology, #8084), p-PSD95 (1:500, Cell Signaling Technology, #45737), total PSD95 (1:500, Cell Signaling Technology, #3409), BDNF (1:300, Santa Cruz Biotechnology, sc-546), and β-actin (1:30000, Sigma-Aldrich, A1978).. Then, the membrane was incubated with the secondary horseradish peroxidase-conjugated goat anti-rabbit antibody (Pierce, Rockford, IL, USA) or mouse (Thermo Scientific; PA1-30355) antibodies.Then, the membrane was incubated..

    Enzyme-linked Immunosorbent Assay:

    Article Title: The effects of different intensities of aerobic exercise for 8 weeks on neurogenesis, depression, and anxiety in young mice
    Article Snippet: For immunodetection, the membranes were blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween-20 (TBS-T) at room temperature for one hour, followed by an overnight incubation at 4°C with primary antibodies.in Tris-buffered saline with 0.1% Tween-20 (TBS-T) at room temperature for one hour, followed by an overnight incubation at 4°C with primary antibodies. ... The primary antibodies used were: p-Akt (1:1,000, #4058), total Akt (1:1,000, #4691), p-ERK1/2 (1:3,000, #4370), total ERK1/2 (1:3,000, #9102), p-CREB (1:1,000, #9198), total CREB (1:1,000, #9197), p-AMPK (1:1,000, #2537), total AMPK (1:1,000, #2532), p-PSD95 (1:1,000, #45737), and total PSD95 (1:1,000, #3409) from Cell Signaling Technology (Danvers, MA, USA); p-Synapsin1 (S9, 1:1,000, ab76260), p-Synapsin1 (S549, 1:1,000, ab254035), p-Synapsin1 (S603, 1:1,000, ab13879), total Synapsin1 (1:1,000, ab64581), and DCX (1:1,000, ab18723) from Abcam (Cambridge, MA); and β-actin (1:30,000, #A1978) from Sigma-Aldrich.. After three 10- min washes with TBS-T at room temperature, the membranes were incubated with secondary antibodies conjugated to horseradish peroxidase, either goat anti-rabbit (Pierce, Rockford, IL, USA) or anti-mouse (Thermo Scientific; 31430).After three 10- min washes with TBS-T at room temperature, the membranes were incubated with secondary antibodies conjugated to horseradish peroxidase, either goat..

    Article Title: Intranasal Administration of Melanin-Concentrating Hormone Reduces Stress-Induced Anxiety- and Depressive-Like Behaviors in Rodents
    Article Snippet: For immunodetection, the blots were blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) for 1 h at room temperature and incubated overnight at 4°C with primary antibodies.For immunodetection, the blots were blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) for 1 h at room temperature and incubated overnight at 4°C with primary antibodies.. The following primary antibodies were used: p-Akt (1:1,000, #4058), total Akt (1:1,000, #4691), p-ERK1/2 (1:3,000, #4370), total ERK1/2 (1:3,000, #9102), p-mTOR (1:500, #2971), total mTOR (1:500, #2983), p-p70S6K (1:500, #9204), p-PSD95 (1:1,000, #45737), total PSD95 (1:1,000, #3409), p-GluR1 (1:1,000, #8084), total GluR1 (1:500, #13185), p-Synapsin1 (p-Syn1, 1:1,000, #88246), and total Syn1 (1:1,000, #9297) from Cell Signaling Technology (Danvers, MA, USA) and β-actin (1:30,000, #A1978) from Sigma-Aldrich.. After washing with TBS-T three times (10 min per wash) at room temperature, the membrane was incubated with secondary horseradish peroxidase-conjugated goat anti-rabbit (Pierce, Rockford, IL, USA) or anti-mouse (Thermo Scientific; 31430) antibodies.After washing with TBS-T three times (10 min per wash) at room temperature, the membrane was incubated with secondary horseradish peroxidase-conjugated goat anti-rabbit (Pierce, Rockford, IL, USA) or anti-mouse (Thermo Scientific;..

    Article Title: Acupuncture modulates stress response by the mTOR signaling pathway in a rat post-traumatic stress disorder model
    Article Snippet: The membrane was blocked in 5% skim milk in Tris buffered saline containing 0.1% Tween-20 (TBS-T) and incubated with the primary antibodies overnight at 4 °C.antibodies overnight at 4 °C. ... The primary antibodies: p-Akt (1:1000, Cell Signaling Technology, #4058), total Akt (1:1000, Cell Signaling Technology, #4691), p-ERK1/2 (1:3000, Cell Signaling Technology, #4370), total ERK1/2 (1:3000, Cell Signaling Technology, #9102), p-mTOR (1:500, Cell Signaling Technology, #2971), total mTOR (1:500, Cell Signaling Technology, #2983), p-CREB (1:500, Cell Signaling Technology, #9198), total CREB (1:500, Cell Signaling Technology, #9197), p-4E-BP-1 (1:500, Cell Signaling Technology, #9456), p-p70S6K (1:500, Cell Signaling Technology, #9204), p-Syn1 (1:500, Cell Signaling Technology, #88246), p-GluR1 (1:500, Cell Signaling Technology, #8084), p-PSD95 (1:500, Cell Signaling Technology, #45737), total PSD95 (1:500, Cell Signaling Technology, #3409), BDNF (1:300, Santa Cruz Biotechnology, sc-546), and β-actin (1:30000, Sigma-Aldrich, A1978).. Then, the membrane was incubated with the secondary horseradish peroxidase-conjugated goat anti-rabbit antibody (Pierce, Rockford, IL, USA) or mouse (Thermo Scientific; PA1-30355) antibodies.Then, the membrane was incubated..

    Phospho-proteomics:

    Article Title: The effects of different intensities of aerobic exercise for 8 weeks on neurogenesis, depression, and anxiety in young mice
    Article Snippet: For immunodetection, the membranes were blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween-20 (TBS-T) at room temperature for one hour, followed by an overnight incubation at 4°C with primary antibodies.in Tris-buffered saline with 0.1% Tween-20 (TBS-T) at room temperature for one hour, followed by an overnight incubation at 4°C with primary antibodies. ... The primary antibodies used were: p-Akt (1:1,000, #4058), total Akt (1:1,000, #4691), p-ERK1/2 (1:3,000, #4370), total ERK1/2 (1:3,000, #9102), p-CREB (1:1,000, #9198), total CREB (1:1,000, #9197), p-AMPK (1:1,000, #2537), total AMPK (1:1,000, #2532), p-PSD95 (1:1,000, #45737), and total PSD95 (1:1,000, #3409) from Cell Signaling Technology (Danvers, MA, USA); p-Synapsin1 (S9, 1:1,000, ab76260), p-Synapsin1 (S549, 1:1,000, ab254035), p-Synapsin1 (S603, 1:1,000, ab13879), total Synapsin1 (1:1,000, ab64581), and DCX (1:1,000, ab18723) from Abcam (Cambridge, MA); and β-actin (1:30,000, #A1978) from Sigma-Aldrich.. After three 10- min washes with TBS-T at room temperature, the membranes were incubated with secondary antibodies conjugated to horseradish peroxidase, either goat anti-rabbit (Pierce, Rockford, IL, USA) or anti-mouse (Thermo Scientific; 31430).After three 10- min washes with TBS-T at room temperature, the membranes were incubated with secondary antibodies conjugated to horseradish peroxidase, either goat..

    Article Title: Intranasal Administration of Melanin-Concentrating Hormone Reduces Stress-Induced Anxiety- and Depressive-Like Behaviors in Rodents
    Article Snippet: For immunodetection, the blots were blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) for 1 h at room temperature and incubated overnight at 4°C with primary antibodies.For immunodetection, the blots were blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) for 1 h at room temperature and incubated overnight at 4°C with primary antibodies.. The following primary antibodies were used: p-Akt (1:1,000, #4058), total Akt (1:1,000, #4691), p-ERK1/2 (1:3,000, #4370), total ERK1/2 (1:3,000, #9102), p-mTOR (1:500, #2971), total mTOR (1:500, #2983), p-p70S6K (1:500, #9204), p-PSD95 (1:1,000, #45737), total PSD95 (1:1,000, #3409), p-GluR1 (1:1,000, #8084), total GluR1 (1:500, #13185), p-Synapsin1 (p-Syn1, 1:1,000, #88246), and total Syn1 (1:1,000, #9297) from Cell Signaling Technology (Danvers, MA, USA) and β-actin (1:30,000, #A1978) from Sigma-Aldrich.. After washing with TBS-T three times (10 min per wash) at room temperature, the membrane was incubated with secondary horseradish peroxidase-conjugated goat anti-rabbit (Pierce, Rockford, IL, USA) or anti-mouse (Thermo Scientific; 31430) antibodies.After washing with TBS-T three times (10 min per wash) at room temperature, the membrane was incubated with secondary horseradish peroxidase-conjugated goat anti-rabbit (Pierce, Rockford, IL, USA) or anti-mouse (Thermo Scientific;..

    Article Title: Acupuncture modulates stress response by the mTOR signaling pathway in a rat post-traumatic stress disorder model
    Article Snippet: The membrane was blocked in 5% skim milk in Tris buffered saline containing 0.1% Tween-20 (TBS-T) and incubated with the primary antibodies overnight at 4 °C.antibodies overnight at 4 °C. ... The primary antibodies: p-Akt (1:1000, Cell Signaling Technology, #4058), total Akt (1:1000, Cell Signaling Technology, #4691), p-ERK1/2 (1:3000, Cell Signaling Technology, #4370), total ERK1/2 (1:3000, Cell Signaling Technology, #9102), p-mTOR (1:500, Cell Signaling Technology, #2971), total mTOR (1:500, Cell Signaling Technology, #2983), p-CREB (1:500, Cell Signaling Technology, #9198), total CREB (1:500, Cell Signaling Technology, #9197), p-4E-BP-1 (1:500, Cell Signaling Technology, #9456), p-p70S6K (1:500, Cell Signaling Technology, #9204), p-Syn1 (1:500, Cell Signaling Technology, #88246), p-GluR1 (1:500, Cell Signaling Technology, #8084), p-PSD95 (1:500, Cell Signaling Technology, #45737), total PSD95 (1:500, Cell Signaling Technology, #3409), BDNF (1:300, Santa Cruz Biotechnology, sc-546), and β-actin (1:30000, Sigma-Aldrich, A1978).. Then, the membrane was incubated with the secondary horseradish peroxidase-conjugated goat anti-rabbit antibody (Pierce, Rockford, IL, USA) or mouse (Thermo Scientific; PA1-30355) antibodies.Then, the membrane was incubated..

    Western Blot:

    Article Title: The effects of different intensities of aerobic exercise for 8 weeks on neurogenesis, depression, and anxiety in young mice
    Article Snippet: For immunodetection, the membranes were blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween-20 (TBS-T) at room temperature for one hour, followed by an overnight incubation at 4°C with primary antibodies.in Tris-buffered saline with 0.1% Tween-20 (TBS-T) at room temperature for one hour, followed by an overnight incubation at 4°C with primary antibodies. ... The primary antibodies used were: p-Akt (1:1,000, #4058), total Akt (1:1,000, #4691), p-ERK1/2 (1:3,000, #4370), total ERK1/2 (1:3,000, #9102), p-CREB (1:1,000, #9198), total CREB (1:1,000, #9197), p-AMPK (1:1,000, #2537), total AMPK (1:1,000, #2532), p-PSD95 (1:1,000, #45737), and total PSD95 (1:1,000, #3409) from Cell Signaling Technology (Danvers, MA, USA); p-Synapsin1 (S9, 1:1,000, ab76260), p-Synapsin1 (S549, 1:1,000, ab254035), p-Synapsin1 (S603, 1:1,000, ab13879), total Synapsin1 (1:1,000, ab64581), and DCX (1:1,000, ab18723) from Abcam (Cambridge, MA); and β-actin (1:30,000, #A1978) from Sigma-Aldrich.. After three 10- min washes with TBS-T at room temperature, the membranes were incubated with secondary antibodies conjugated to horseradish peroxidase, either goat anti-rabbit (Pierce, Rockford, IL, USA) or anti-mouse (Thermo Scientific; 31430).After three 10- min washes with TBS-T at room temperature, the membranes were incubated with secondary antibodies conjugated to horseradish peroxidase, either goat..

    Article Title: Intranasal Administration of Melanin-Concentrating Hormone Reduces Stress-Induced Anxiety- and Depressive-Like Behaviors in Rodents
    Article Snippet: For immunodetection, the blots were blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) for 1 h at room temperature and incubated overnight at 4°C with primary antibodies.For immunodetection, the blots were blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) for 1 h at room temperature and incubated overnight at 4°C with primary antibodies.. The following primary antibodies were used: p-Akt (1:1,000, #4058), total Akt (1:1,000, #4691), p-ERK1/2 (1:3,000, #4370), total ERK1/2 (1:3,000, #9102), p-mTOR (1:500, #2971), total mTOR (1:500, #2983), p-p70S6K (1:500, #9204), p-PSD95 (1:1,000, #45737), total PSD95 (1:1,000, #3409), p-GluR1 (1:1,000, #8084), total GluR1 (1:500, #13185), p-Synapsin1 (p-Syn1, 1:1,000, #88246), and total Syn1 (1:1,000, #9297) from Cell Signaling Technology (Danvers, MA, USA) and β-actin (1:30,000, #A1978) from Sigma-Aldrich.. After washing with TBS-T three times (10 min per wash) at room temperature, the membrane was incubated with secondary horseradish peroxidase-conjugated goat anti-rabbit (Pierce, Rockford, IL, USA) or anti-mouse (Thermo Scientific; 31430) antibodies.After washing with TBS-T three times (10 min per wash) at room temperature, the membrane was incubated with secondary horseradish peroxidase-conjugated goat anti-rabbit (Pierce, Rockford, IL, USA) or anti-mouse (Thermo Scientific;..

    Article Title: Acupuncture modulates stress response by the mTOR signaling pathway in a rat post-traumatic stress disorder model
    Article Snippet: The membrane was blocked in 5% skim milk in Tris buffered saline containing 0.1% Tween-20 (TBS-T) and incubated with the primary antibodies overnight at 4 °C.antibodies overnight at 4 °C. ... The primary antibodies: p-Akt (1:1000, Cell Signaling Technology, #4058), total Akt (1:1000, Cell Signaling Technology, #4691), p-ERK1/2 (1:3000, Cell Signaling Technology, #4370), total ERK1/2 (1:3000, Cell Signaling Technology, #9102), p-mTOR (1:500, Cell Signaling Technology, #2971), total mTOR (1:500, Cell Signaling Technology, #2983), p-CREB (1:500, Cell Signaling Technology, #9198), total CREB (1:500, Cell Signaling Technology, #9197), p-4E-BP-1 (1:500, Cell Signaling Technology, #9456), p-p70S6K (1:500, Cell Signaling Technology, #9204), p-Syn1 (1:500, Cell Signaling Technology, #88246), p-GluR1 (1:500, Cell Signaling Technology, #8084), p-PSD95 (1:500, Cell Signaling Technology, #45737), total PSD95 (1:500, Cell Signaling Technology, #3409), BDNF (1:300, Santa Cruz Biotechnology, sc-546), and β-actin (1:30000, Sigma-Aldrich, A1978).. Then, the membrane was incubated with the secondary horseradish peroxidase-conjugated goat anti-rabbit antibody (Pierce, Rockford, IL, USA) or mouse (Thermo Scientific; PA1-30355) antibodies.Then, the membrane was incubated..

    Suspension:

    Article Title: The effects of different intensities of aerobic exercise for 8 weeks on neurogenesis, depression, and anxiety in young mice
    Article Snippet: For immunodetection, the membranes were blocked with 5% skim milk in Tris-buffered saline with 0.1% Tween-20 (TBS-T) at room temperature for one hour, followed by an overnight incubation at 4°C with primary antibodies.in Tris-buffered saline with 0.1% Tween-20 (TBS-T) at room temperature for one hour, followed by an overnight incubation at 4°C with primary antibodies. ... The primary antibodies used were: p-Akt (1:1,000, #4058), total Akt (1:1,000, #4691), p-ERK1/2 (1:3,000, #4370), total ERK1/2 (1:3,000, #9102), p-CREB (1:1,000, #9198), total CREB (1:1,000, #9197), p-AMPK (1:1,000, #2537), total AMPK (1:1,000, #2532), p-PSD95 (1:1,000, #45737), and total PSD95 (1:1,000, #3409) from Cell Signaling Technology (Danvers, MA, USA); p-Synapsin1 (S9, 1:1,000, ab76260), p-Synapsin1 (S549, 1:1,000, ab254035), p-Synapsin1 (S603, 1:1,000, ab13879), total Synapsin1 (1:1,000, ab64581), and DCX (1:1,000, ab18723) from Abcam (Cambridge, MA); and β-actin (1:30,000, #A1978) from Sigma-Aldrich.. After three 10- min washes with TBS-T at room temperature, the membranes were incubated with secondary antibodies conjugated to horseradish peroxidase, either goat anti-rabbit (Pierce, Rockford, IL, USA) or anti-mouse (Thermo Scientific; 31430).After three 10- min washes with TBS-T at room temperature, the membranes were incubated with secondary antibodies conjugated to horseradish peroxidase, either goat..

    Article Title: Intranasal Administration of Melanin-Concentrating Hormone Reduces Stress-Induced Anxiety- and Depressive-Like Behaviors in Rodents
    Article Snippet: For immunodetection, the blots were blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) for 1 h at room temperature and incubated overnight at 4°C with primary antibodies.For immunodetection, the blots were blocked with 5% skim milk in Tris-buffered saline containing 0.1% Tween-20 (TBS-T) for 1 h at room temperature and incubated overnight at 4°C with primary antibodies.. The following primary antibodies were used: p-Akt (1:1,000, #4058), total Akt (1:1,000, #4691), p-ERK1/2 (1:3,000, #4370), total ERK1/2 (1:3,000, #9102), p-mTOR (1:500, #2971), total mTOR (1:500, #2983), p-p70S6K (1:500, #9204), p-PSD95 (1:1,000, #45737), total PSD95 (1:1,000, #3409), p-GluR1 (1:1,000, #8084), total GluR1 (1:500, #13185), p-Synapsin1 (p-Syn1, 1:1,000, #88246), and total Syn1 (1:1,000, #9297) from Cell Signaling Technology (Danvers, MA, USA) and β-actin (1:30,000, #A1978) from Sigma-Aldrich.. After washing with TBS-T three times (10 min per wash) at room temperature, the membrane was incubated with secondary horseradish peroxidase-conjugated goat anti-rabbit (Pierce, Rockford, IL, USA) or anti-mouse (Thermo Scientific; 31430) antibodies.After washing with TBS-T three times (10 min per wash) at room temperature, the membrane was incubated with secondary horseradish peroxidase-conjugated goat anti-rabbit (Pierce, Rockford, IL, USA) or anti-mouse (Thermo Scientific;..

    Article Title: Acupuncture modulates stress response by the mTOR signaling pathway in a rat post-traumatic stress disorder model
    Article Snippet: The membrane was blocked in 5% skim milk in Tris buffered saline containing 0.1% Tween-20 (TBS-T) and incubated with the primary antibodies overnight at 4 °C.antibodies overnight at 4 °C. ... The primary antibodies: p-Akt (1:1000, Cell Signaling Technology, #4058), total Akt (1:1000, Cell Signaling Technology, #4691), p-ERK1/2 (1:3000, Cell Signaling Technology, #4370), total ERK1/2 (1:3000, Cell Signaling Technology, #9102), p-mTOR (1:500, Cell Signaling Technology, #2971), total mTOR (1:500, Cell Signaling Technology, #2983), p-CREB (1:500, Cell Signaling Technology, #9198), total CREB (1:500, Cell Signaling Technology, #9197), p-4E-BP-1 (1:500, Cell Signaling Technology, #9456), p-p70S6K (1:500, Cell Signaling Technology, #9204), p-Syn1 (1:500, Cell Signaling Technology, #88246), p-GluR1 (1:500, Cell Signaling Technology, #8084), p-PSD95 (1:500, Cell Signaling Technology, #45737), total PSD95 (1:500, Cell Signaling Technology, #3409), BDNF (1:300, Santa Cruz Biotechnology, sc-546), and β-actin (1:30000, Sigma-Aldrich, A1978).. Then, the membrane was incubated with the secondary horseradish peroxidase-conjugated goat anti-rabbit antibody (Pierce, Rockford, IL, USA) or mouse (Thermo Scientific; PA1-30355) antibodies.Then, the membrane was incubated..



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